Molecular Biology
Full course description
The general aim of this skills course is to obtain detailed knowledge about the techniques that can be applied to address molecular processes in mammalian biology. Topics include intracellular signalling pathways and nucleic acid vs protein interactions, plus the culmination of the above elements in a grant proposal assignment to indicate active understanding of the above processes. The skills days are designed to provide a perspective of how molecular biological techniques are applied to tackle major research questions in modern biomedical research.
Course objectives
- To be able to investigate protein/nucleic acid interactions via an electrophoretic mobility shift assay (EMSA);
- To apply basic cloning tools in order to manipulate nucleic acids;
- To isolate proteins from cells and perform protein quantification;
- To perform an immunoprecipitation (IP) and interpret the final results by Western immunoblotting;
- To put together a research grant proposal plan based on previously published research; to present and defend it towards peers and tutors.
Prerequisites
- PRA2014
-
1000-level core courses
Co-requisites
- BIO3001
Recommended reading
A reader is provided at the start of the course, containing concomitant additional literature sources.
PRA3003
Period 2
28 Oct 2024
20 Dec 2024
Period 5
7 Apr 2025
6 Jun 2025
ECTS credits:
2.5Instruction language:
EnglishCoordinator:
- T.D. Steen Redeker
Teaching methods:
Skills, Work in subgroupsAssessment methods:
Attendance, Presentation, Written exam