Applied Cell Biology

Applied Cell Biology

Full course description

These skills will contain:

  • Immunocytochemistry (ICC) on HIF translocation in hypoxia/Phenantroline treated and untreated cells;
  • RT-qPCR using Sybr Green for anti- and pro-apoptotic markers;
  • Metabolic assays (PrestoBlue assay);
  • Microscopy;
  • Computational analysis of obtained data (Use of CellProfiler to analyze ICC data).

Course objectives

The main objective of this course is to provide a practical introduction to molecular and cell biology. We will use osteoblasts to study the effects of a hypoxia mimic compound on programmed cell death. A variety of experiments will be performed to study the effects on RNA and protein levels. Cell Profiler will be used for image analysis.

Prerequisites

  • PRA2014

Co-requisites

  • None

Recommended reading

  • Course manual.
  • Oxygen-distribution within 3-D collagen I hydrogels for bone tissue engineering (P Wolff, Materials Science & Engineering C95 (2019) 422-427).
PRA3017
Period 4
3 Feb 2025
4 Apr 2025
Print course description
ECTS credits: 
2.5
Instruction language: 
English
Coordinator:
  • S. Bolognin
Teaching methods: 
Skills, Assignment(s)
Assessment methods: 
Attendance, Assignment, Written exam