Practical Mass Spectrometry
Full course description
This course focusses on gaining practical experience with a variety of MS and ionization technologies, including MALDI, ESI and APPI. Next insight into the Ion Mobility Spectrometry (IMS) will be gained and appliued for the structural identification of biological relevant molecules like, proteins or small molecule hormones. The optimization of ESI, by varying e.g. ion-focusing lenses and electrical field strength, will give the student an introduction in the practical aspects of ion-physics. Likewise, optimization of MS/MS methodologies will allow the students to recognize the importance of the physical foundations of gas- phase molecular collisions, the assessment of molecular structures. By developing MS imaging methodologies, physical chemical processes at the surface of tissues or biomedical materials will be gained. As MS plays an essential role in many R&D laboratories, a broad field of applications, including human tissues, endogenous compounds such as lipids and proteins, pharmaceutical drugs, biomedical materials such as orthopedic implants will be investigated.
Course objectives
- Familiarization and practical experience with ionization techniques; ESI, MALDI and APCI;
- Basic understanding of ion physics and ion trajectories in mass spectrometers;
- Basic training in the application of tandem mass spectrometry (MS/MS) for biology and chemistry relevant molecules, the use of MS/MS prediction software;
- Ability to utilize ion mobility spectrometry (IMS) for molecular structure analysis;
- Understanding of the assessment of the native structures of proteins by MS;
- Basic understanding on the application of vacuum systems, optimization of detectors, ion optics, electronics.
Prerequisites
- INT2010
Co-requisites
- None
Recommended reading
Mass Spectrometry Principles and Applications, de Hoffman, power point slides, articles to be distributed. Students are requested to search literature for specific manuscripts.