Practical Training: Cell Culture
Full course description
The practical ‘Introduction to basic cell culture procedures’ compromises a hands-on training, where each student will maintain his/her own cell culture, and will receive training on aseptic techniques and basic cell culture procedures. To this end, a human cell line will be used for practice. Various topics will be tackled, including aseptic techniques, thawing of cells, cell passage, cell counting, and cryopreservation of cells. The practical includes an introductory lecture and a workshop where (advanced) cell models of pain are presented to the students. Before coming to the practical session, the students are required to have completed a self-study exercise. The self-study exercise will be assessed by the supervisor(s) and discussed in subgroups before the start of each practical session.
Course objectives
Students will be able to understand:
- Asceptic techniques;
- Basic cell culture procedures;
- (Advanced) in vitro models of pain;
- Translational pain modelling.