Genetics

Volledige vakbeschrijving

The skills trainings are aimed to obtain a basic introduction to techniques and methods in modern Genetics. The first skills take place at a designated skills laboratory at Chemelot campus; subsequent skills training topics "Genomes and Genomics" and are taught in a computer landscape. These days integrate theoretical and practical information. Each student will receive theoretical and practical in silico training in the morning, followed by a limited number of tasks to execute on the computer and answered in a skills report. The final skills consist of a student group presentation where the combined theoretical and practical skills on Genetics are applied to a pre-assigned task.

Doelstellingen van dit vak

• To be able to purify genomic DNA from eukaryotic cells and plasmid (circular) DNA from prokaryotic cells and perform quantitative analyses on each product. • To perform and comprehend polymerase chain reaction (PCR) analysis. • Analyze DNA products using restriction digestion, ligation and agarose gel electrophoresis. • To isolate RNA from eukaryotic cells and apply reverse transcription to generate copy DNA • To study specific proteins by Western immunoblotting. • To independently use genetic and genomic websites, general and specialized databases and determine relationships of genes within and between databases. • To have sufficient technical training for more advanced skills in molecular biology and the life sciences. • To apply genetic principles to a pre-assigned task and present the findings to a larger audience.

Voorwaarden

Pre-requisites BIO2001 Cell Biology Co-requisites BIO2007 Genetics

Aanbevolen literatuur

"Introduction to Genetic Analysis" by Griffiths, Wessler, Carrol, Doebley (W.H. Freeman, 10thedition, International Edition, 2012. bol.com price €64,99).

PRA2014

Periode 1

2 sep 2024

25 okt 2024

Periode 4

3 feb 2025

4 apr 2025

Vakbeschrijving afdrukken

Studiepunten:

2.5

Taal van de opleiding:

Engels

Coordinators:

Onderwijsmethode:

PBL, Research, Skills, Work in subgroups, Assignment(s)

Evaluatiemethoden:

Participation, Assignment, Attendance