Advanced Microscopy: Theory and Applications

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The use of advanced imaging techniques in light and electron microscopy is wide-spread, particularly in the fields of material science and biological imaging. In recent years, the resolution and/or functionality of such tools has been increased due to the development of fluorescence microscopy, two-photon excitation microscopy, correlative light-electron microscopy and stimulation emission depletion (STED) microscopy [first experimentally shown by the winners of the 2014 Nobel Prize in Chemistry]. In electron microscopy, rapid developments in aberration correction and in image filtering allow users to understand much more about the samples they are investigating. This course will introduce a number of advanced imaging techniques to participants, detailing theoretical aspects as well as practical considerations. This course is aimed any students from the programme with an interest in imaging and its principles.